A critical study on the interactions of hesperitin with human hemoglobin: Fluorescence spectroscopic and molecular modeling approach

► Absorption spectra of hesperitin bound HbA indicates ground state complex formation. ► Binding induces static quenching of intrinsic fluorescence of the tryptophan of HbA. ► Molecular docking and electrostatic surface potential calculations were performed. ► Contrasting binding modes of hesperitin compared to other flavonoids were observed.