Study of the interaction between tosufloxacin tosylate and bovine serum albumin by multi-spectroscopic methods

► Interaction of TSFX and BSA was studied by multi-spectroscopic methods. ► Binding action was static quenching mainly by electrostatic interactions. ► Binding constant, number of binding sites and binding distance were calculated. ► TSFX changed the conformation and secondary structure of BSA. ► With TSFX the polarity of Trp micro-region increased and the polypeptides unfolded.