Spectroscopic and voltammetric characterizations of the interaction of two local anesthetics with bovine serum albumin

► Procaine or tetracaine hydrochloride quenching the fluorescence of Trp in BSA was a static quenching process. ► Synchronous fluorescence was applied to study the structural change of BSA. ► Binding constant, binding site and binding force were determined. ► Voltammetry techniques further characterized the nature of the interactions of the two drugs with BSA.