کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5402273 | 1505928 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Self-quenching of uranin: Instrument response function for color sensitive photo-detectors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
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چکیده انگلیسی
Concentration is a key determining factor in the fluorescence properties of organic fluorophores. We studied self-quenching of disodium fluorescein (uranin) fluorescence in polyvinyl alcohol (PVA) thin films. The concentration dependent changes in brightness and anisotropy were followed by a lifetime decrease. We found that at a concentration of 0.54Â M, the lifetime decreases to 7Â ps. At a concentration of 0.18Â M the lifetime was 10Â ps with the relatively high quantum yield of 0.002. In these conditions the fluorescence intensity decay was homogeneous (well approximated by a single lifetime). We realized that such a sample was an ideal fluorescence lifetime standard for spectroscopy and microscopy, and therefore characterized instrument response functions for a time-domain technique. We show that self-quenched uranin enables measurements free of the color effect, making it a superior choice for a lifetime reference over scattered light.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 130, Issue 12, December 2010, Pages 2446-2451
Journal: Journal of Luminescence - Volume 130, Issue 12, December 2010, Pages 2446-2451
نویسندگان
Rafal Luchowski, Sushant Sabnis, Mariusz Szabelski, Pabak Sarkar, Sangram Raut, Zygmunt Gryczynski, Julian Borejdo, Piotr Bojarski, Ignacy Gryczynski,