کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5402566 | 1392737 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluorimetric study of interaction of benzidine with trypsin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The interaction of benzidine (BEN) with trypsin was studied by fluorescence spectrum. It was shown that BEN has quenched the fluorescence launching from trypsin by reacting with it and forming a certain kind of new complex. The quenching and energy transfer mechanisms were discussed. The binding constants and thermodynamic parameters at three different temperatures, the binding locality, and the binding power were obtained. The conformation of trypsin was discussed by synchronous and three-dimensional fluorescence techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 130, Issue 5, May 2010, Pages 781-786
Journal: Journal of Luminescence - Volume 130, Issue 5, May 2010, Pages 781-786
نویسندگان
Hong-Mei Zhang, Yan-Qing Wang, Qiu-Hua Zhou,