کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5402785 | 1392741 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Concentration dependence of fluorescence signal in a microfluidic fluorescence detector
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Fluorescence detection is one of the most widely used techniques for measuring analyte concentration in biological applications. Although used frequently with bulk systems, there are important considerations in using this approach in thin (<100 μm) microfluidic lab-on-a-chip (LOC) systems in relating the measured fluorescence signal to analyte concentration. In this work, a general relationship between the photoresponse of an on-chip organic photodetector (OPD) and concentration of a fluorescent dye Rhodamine 6G in a microfluidic chip is presented. The developed theoretical model for photoresponse matches well to the extreme sub-linearity observed in measurements in a dilution series from 10 nM to 1 mM. The influence of the system factors, such as noise, detector sensitivity, and optical power were also analyzed to indicate design changes to improve the system performance and limits of detection even further.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 130, Issue 6, June 2010, Pages 1095-1100
Journal: Journal of Luminescence - Volume 130, Issue 6, June 2010, Pages 1095-1100
نویسندگان
Ansuman Banerjee, Yun Shuai, Rahul Dixit, Ian Papautsky, David Klotzkin,