کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5402799 | 1392742 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Luminescence quenching effect for the interaction of prulifloxacin with trypsin-Britton-Robinson buffer solution system
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Prulifloxacin is a kind of new oral taking antibiotic of fluoroquinolone. Conjugation reaction of prulifloxacin with trypsin in Britton-Robinson buffer solution of pH 7.96 was analyzed by UV-vis spectrophotometry and fluorescence spectrometry. The intrinsic fluorescence of trypsin was strongly quenched by prulifloxacin. This effect was rationalized in terms of a static quenching procedure. The binding parameters have been evaluated by fluorescence quenching methods. Negative values ÎG0 for the formation of prulifloxacin-trypsin complex implied that both hydrogen bonds and hydrophobic interactions might play a significant role in prulifloxacin binding to trypsin. The binding distance deduced from the efficiency of energy transfer was 0.84Â nm for prulifloxacin-trypsin. Furthermore, association constants and binding mechanism were successfully derived from the fluorescence spectra. UV-vis detections supported a change in the secondary structure of proteins caused by the interaction of prulifloxacin with trypsin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 130, Issue 3, March 2010, Pages 360-364
Journal: Journal of Luminescence - Volume 130, Issue 3, March 2010, Pages 360-364
نویسندگان
Yabei Huang, Benzhi Liu, Zhang Yu, Yanqin Zi,