کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5403470 1392760 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study on the binding of 2,3-diazabicyclo[2.2.2]oct-2-ene with bovine serum albumin by fluorescence spectroscopy
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Study on the binding of 2,3-diazabicyclo[2.2.2]oct-2-ene with bovine serum albumin by fluorescence spectroscopy
چکیده انگلیسی
The interaction of 2,3-diazabicyclo[2.2.2]oct-2-ene (DBO) with bovine serum albumin (BSA) has been studied using absorption and steady state fluorescence techniques. Fluorescence spectrum of BSA (λexi=280 nm) in the presence of DBO clearly shows that DBO acts as a quencher. The number of binding sites 'n' and apparent binding constant 'K' were measured by Stern-Volmer equation. Synchronous fluorescence and absorption spectra were used to study protein conformation. The interaction between DBO and BSA is consistent with static quenching and the conformational changes of BSA observed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 128, Issue 9, September 2008, Pages 1454-1458
نویسندگان
, ,