کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
540359 | 871311 | 2011 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impedance monitoring of cell adhesion and growth on mesoporous membrane
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی کامپیوتر
سخت افزارها و معماری
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
We present a microdevice configuration for impedance monitoring of adherent cells cultured on the surface of mesoporous membranes. The device fabrication was based on a 3 layer PDMS process which allows easy integration of microelectrodes in close proximity to cultured cells. The measurements were performed with HeLa cells on 30 nm diameter membranes of polycarbonate and embedded Pt and Ag/AgCl electrodes. As expected, the impedance of the cell-membrane assembly changes rapidly during the first 30 min due to cell adhesion and it continuously increases until cell confluence at day 5. This membrane based device configuration should be useful for accurate and real-time monitoring in cell adhesion, growth and migration studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microelectronic Engineering - Volume 88, Issue 8, August 2011, Pages 1722–1725
Journal: Microelectronic Engineering - Volume 88, Issue 8, August 2011, Pages 1722–1725
نویسندگان
Lianmei Jiang, Jianmiao Liu, Jian Shi, Xin Li, Hao Li, Jun Liu, Jiannong Ye, Yong Chen,