Determination of adenosine disodium triphosphate (ATP) using norfloxacin-Tb3+ as a fluorescence probe by spectrofluorimetry

A new spectrofluorimetric method was developed for determination of trace amount of adenosine disodium triphosphate (ATP). Using norfloxacin (NFLX)-terbium (Tb3+) as a fluorescent probe, in the buffer solution of pH=7.40, ATP can remarkably enhance the fluorescence intensity of the NFLX-Tb3+ complex at λ=545nm and the enhanced fluorescence intensity of Tb3+ ion is in proportion to the concentration of ATP. Optimum conditions for the determination of ATP were also investigated. The dynamic range for the determination of ATP is 1.00×10−6-1.60×10−5 mol/L with detection limit of 4.13×10−8 mol/L. This method is simple, practical and relatively free interference from coexisting substances and can be successfully applied to determination of ATP in samples.