Poly(ionic liquid) immobilized magnetic nanoparticles as sorbent coupled with fluorescence spectrophotometry for separation/analysis of Allura red

Poly(ionic liquid)immobilized magnetic nanoparticles (PIL-MNPs) sorbent were introduced for magnetic solid phase extraction(MSPE)accompanied with fluorescence spectrophotometry for Allura red (AR) separation in food samples. Poly (1-vinyl-3- butylimidazolium) bromide immobilized (MNPs) were prepared through co-polymerization of 1-vinyl-3-hexylimidazolium-based ionic liquid and vinyl-modified magnetic particles. The PIL-MNPs were used as adsorbent phases in MSPE. Different parameters, like pH value, adsorption temperature and time, sample volume, eluent type, volume and time were studied. This method introduced the linearity for AR in concentration range between 0.01 and 0.50 μg mL, correlation coefficient (R) was 0.9993 and the limit of detection was found to be 0.002 μg mL. the equations of calibration graph was A (absorbance) = 0.04 + 0.13c (μg mL− 1).This method was successfully applied for separation/analysis of Allura red pigment in candies and beverages samples with satisfactory results.