کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5413801 | 1393497 | 2006 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of horseradish peroxidase activity by thiol type inhibitors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Inhibition of horseradish peroxidase activity by thiol type inhibitors Inhibition of horseradish peroxidase activity by thiol type inhibitors](/preview/png/5413801.png)
چکیده انگلیسی
The inhibition by cysteine and glutathione of 4-amino antipyrine oxidation by horseradish peroxidase was compared. The kinetics of the peroxidase inhibition by glutathione was investigated and compared to l-cysteine inhibition. IC50 for l-cysteine and glutathione was determined and used for the subsequent kinetics studies. The biological activity of the enzyme was measured in the presence of each inhibitor using 4-amino antipyrine as substrate. It was found that reduced glutathione was a more potent inhibitor on peroxidase activity than l-cysteine. Kinetic studies showed that inhibition was non-competitive and mixed type in both cases. The values of Km and Vmax in the presence of each inhibitor were also calculated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Liquids - Volume 123, Issue 1, 15 January 2006, Pages 20-23
Journal: Journal of Molecular Liquids - Volume 123, Issue 1, 15 January 2006, Pages 20-23
نویسندگان
R. Sariri, R.H. Sajedi, V. Jafarian,