کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5425074 | 1395847 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation of oligonucleotide microarrays on modified glass using a photoreactive heterobifunctional reagent, 1-N-(maleimidohexanoyl)-6-N-(anthraquinon-2-oyl) hexanediamine (MHAHD)
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کلمات کلیدی
NHSHeterobifunctional reagentN-methylimidazoledicyclohexylcarbodiimideN-hydroxysuccinimideDCCTLCTHFDMSO - DMSONmi - NMIImmobilization - ایمن سازیTetrahydrofuran - تتراهیدروفورانDimethylsulfoxide - دیمتیل سولفواکسیدMicrowaves - مایکروویو هاOligonucleotide microarrays - میکروارگانیهای OligonucleotideMismatches - ناسازگاریHybridization - هیبریداسیونTLC, Thin layer chromatography - کروماتوگرافی لایه نازک
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel approach for the construction of oligonucleotide microarrays under the influence of light and microwaves is described. For that purpose, a new heterobifunctional crosslinking reagent, 1-N-(maleimidohexanoyl)-6-N-(anthraquinon-2-oyl) hexanediamine (MHAHD), possessing a photoactive anthraquinone moiety on one terminus and an electrophilic maleimide group on the other one, was developed. The immobilization of oligonucleotides using the reagent, MHAHD, was realized via two routes (A and B). In order to speed up the immobilization procedure, the reaction between 3â²- or 5â²-mercaptoalkylated oligonucleotides and maleimide moiety of MHAHD was carried out under microwaves in just 15 min. The oligonucleotide arrays produced by both the routes were analyzed by hybridization experiments (hybridization efficiency 30.13%) and subsequently used for the discrimination of base mismatches. The constructed microarrays were found to possess good thermal stability (only â¼4.5% loss of fluorescence intensity observed after ten cycles).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Surface Science - Volume 602, Issue 14, 15 July 2008, Pages 2389-2394
Journal: Surface Science - Volume 602, Issue 14, 15 July 2008, Pages 2389-2394
نویسندگان
Jyoti Choithani, Dalip Sethi, P. Kumar, Kailash C. Gupta,