Raman spectroscopy for detection of stretched DNAs on superhydrophobic surfaces

• Superhydrophobic surface constituted with micropillars.
• Single strand DNA and dsDNA spectroscopic analysis.
• Low concentrated biomolecules Raman analysis.
• Non-enhanced Raman spectroscopy for DNAs molecules.
• Distinction of ss and dsDNAs via Raman spectroscopy.

A novel approach for the study of low concentrated DNAs (60 pM) using microRaman spectroscopy is reported. A superhydrophobic substrate with array of microPillars is fabricated over which the sample was drop casted. The substrate concentrates the molecules in a very small area with higher molecular density, enabling to carry out the microRaman measurements. Two different DNAs (single strand and double strand) were used to investigate through Raman technique. A spectral Raman difference was found to distinguish the ssDNA and dsDNAs. The approach can be of interest for a wide variety of applications ranging from biological materials interactions characterization to the biomedical field.

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