کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5434097 1509006 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The interaction of protamine nanocapsules with the intestinal epithelium: A mechanistic approach
ترجمه فارسی عنوان
تعامل پروتامین نانوکپسول با اپیتلیوم روده: رویکرد مکانیکی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی

Single-layer protamine and double layer polysialic acid (PSA)/protamine nanocapsules (NCs) were designed in order to be used as carriers to facilitate the transport of macromolecules across the intestinal epithelium. The rational for the design of these NCs was based on that protamine is a non-toxic yet potent cell-penetrating peptide, capable of translocating protein cargos through cell membranes, while PSA is a low molecular weight polysaccharide used to enhance the stability of macromolecules and nanocarriers. The aim of this work was to study in vitro the mechanism of interaction of these NCs with different intestinal cell models (Caco-2, Caco-2/Raji mimicking follicle associated epithelium and Caco-2/HT29-MTX to study the effect of mucus). For this, a fluorescent marker, TAMRA was covalently linked to protamine. The interaction and transport of the NCs with the Caco-2 cells was found to be concentration, temperature and size dependent. In all cases, the double layer PSA-protamine NCs exhibited a significantly higher transport compared to protamine NCs. On the other hand, the transport of the NCs was significantly higher in the co-culture (Caco-2/Raji monolayer) compared to the monoculture model (Caco-2 monolayer), implying that M cells are involved in the transport of these nanosystems. The formulations, administered intra-jejunally to healthy rats (4 h fasting) resulted in a moderate reduction of the glucose levels (20% reduction), which lasted for up to 4 h. This work raises prospects that protamine-based nanocapsules may have the potential as oral peptide delivery nanocarriers.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 243, 10 December 2016, Pages 109-120
نویسندگان
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