|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5434422||1509143||2017||8 صفحه PDF||سفارش دهید||دانلود رایگان|
- Graphene oxide nanoribbons (O-GNR) were obtained from multi-walled carbon nanotubes.
- High amounts of O-GNR were evaluated to identify the dose-dependence for biomedical applications
- O-GNR neither showed cytotoxic effects nor promoted gene expression alteration up to a concentration of 100Â Î¼gÂ mLâÂ 1.
- O-GNR promoted 50% of S. aureus and E. coli death at 100Â Î¼gÂ mLâÂ 1.
Graphene oxide nanoribbons (O-GNR) surges as an interesting nanomaterial for biomedical applications due to feasibility to incorporate functional groups and possible bactericidal properties. Herein, high concentrations of O-GNR were biologically evaluated using human osteoblast cells and gram positive and negative bacteria. Briefly, our goal were to evaluate: (1) synthetic pathway, (2) characterization and (3) effects of O-GNR composition and structural factors as a new approach for biomedical applications. For this, O-GNR were produced combining chemical vapor deposition and oxygen plasma treatment of multiwalled carbon nanotubes. Then, we analyzed the bioactivity, cell viability, osteogenic differentiation, matrix mineralization, mRNA levels of the five genes related direct to bone repair and bactericidal effect of high concentrations of O-GNR (10Â Î¼gÂ mLâÂ 1, 100Â Î¼gÂ mLâÂ 1, 200Â Î¼gÂ mLâÂ 1 and 300Â Î¼gÂ mLâÂ 1). Impressively, O-GNR showed no cytotoxic effects up to a concentration of 100Â Î¼gÂ mLâÂ 1 and no gene expression alteration when used in its dose. We also observed that S. aureus and E. coli bacteria are susceptible to damage when incubated with 100Â Î¼gÂ mLâÂ 1 of O-GNR, showing approximately 50% of bacterial death. We consider that O-GNR displays attractive properties when used at a suitable dose, displaying bactericidal effect and apparently lacking to cause damages in the bone repair process.
Journal: Materials Science and Engineering: C - Volume 78, 1 September 2017, Pages 341-348