|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5435199||1509149||2017||10 صفحه PDF||سفارش دهید||دانلود رایگان|
- Surface modified PTX-NCs with HA and Tf are successfully prepared by adsorption method.
- Enhanced cellular uptake of modified PTX-NCs compared to unmodified PTX-NC
- Improved cell killing effect by surface modified PTX-NCs
To overcome the toxicity of excipient or blank nanoparticles for drug delivery nano-system, the surface modified paclitaxel nanocrystals (PTX-NC) have been developed. PTX-NCs were prepared by nano-precipitation method. The surface of PTX-NCs were modified by grafting with apo-transferrin (Tf) or hyaluronic acid (HA). The physical properties of PTX-NCs were evaluated by field emission scanning electron microscope (FE-SEM), zeta-sizer, zeta-potential, differential scanning calorimetry (DSC) and Fourier transform infrared (FT-IR) spectrometry. In vitro drug release study was performed in phosphate buffered saline (PBS) with or without 0.5% (w/v) Tween 80 for 24Â h. Cellular uptake was studied at time intervals of 0.5, 1, and 2Â h in MCF-7 cells, and cell growth inhibition study was performed for 24Â h using MCF-7 cells (cancer cells), and HaCaT cells (normal cells).Three different types of PTX-NCs with a mean size of 236.0Â Â±Â 100.6Â nm (PTX-NC), 302.0Â Â±Â 152.0Â nm (Tf-PTX-NC) and 339Â Â±Â 180.6Â nm (HA-PTX-NC) were successfully prepared. The drug release profiles showed 29.1%/6.9% (PTX (pure)), 40.7%/23.9% (PTX-NC), 50.5%/25.1% (Tf-PTX-NC) and 46.8/24.8% (HA-PTX-NC) in PBS with/without 0.5% (w/v) Tween 80 for 24Â h, respectively. As per the results, the drug release of PTX-NCs showed the faster release as compared to that of PTX (pure). Surface modified PTX-NCs exhibited higher values for cell permeability than unmodified PTX-NC in the cellular uptake study. Surface modified PTX-NCs inhibited the cell growth approximately to 60% in MCF-7 cells, however effect of surface modified PTX-NCs on normal cell line was lower than the PTX-NC and PTX (pure). In conclusion, biological macromolecules (Tf or HA) surface modified PTX-NC enhanced the cellular uptake and the cell growth inhibition.
Journal: Materials Science and Engineering: C - Volume 72, 1 March 2017, Pages 228-237