کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5485652 1399435 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inactivation of Planktonic Escherichia coli by Focused 2-MHz Ultrasound
ترجمه فارسی عنوان
غیر فعال کردن اشرشیاکلی پلانکتون توسط اولتراسوند متمرکز 2 مگاهرتز
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم آکوستیک و فرا صوت
چکیده انگلیسی
This study was motivated by the desire to develop a non-invasive means to treat abscesses, and represents the first steps toward that goal. Non-thermal, high-intensity focused ultrasound (HIFU) was used to inactivate Escherichia coli (∼1 × 109 cells/mL) in suspension. Cells were treated in 96-well culture plate wells using 1.95-MHz ultrasound and incident focal acoustic pressures as high as 16 MPa peak positive and 9.9 MPa peak negative (free field measurements). The surviving fraction was assessed by coliform culture and the alamarBlue assay. No biologically significant heating was associated with ultrasound exposure. Bacterial inactivation kinetics were well described by a half-life model, with a half-time of 1.2 min. At the highest exposure levels, a 2log inactivation was typically achieved within 10 min. The free field-equivalent peak negative acoustic pressure threshold for inactivation was ∼7 MPa. At the highest acoustic pressures used, inactivation efficacy was insensitive to reciprocal changes in pulse length and pulse repetition frequency at constant duty factor. Although treated volumes were very small, proof of principle was provided by these experiments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasound in Medicine & Biology - Volume 43, Issue 7, July 2017, Pages 1476-1485
نویسندگان
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