کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5503637 | 1535593 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of survival-promoting OSIP108 peptide variants and their internalization in human cells
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کلمات کلیدی
SAR3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide - 3- (4،5-dimethyl-2-thiazolyl) -2،5-difenyl-2H-tetrazolium bromideAUC - AUCMTT - MTTSurvival - بقاtrypan blue - تریپان آبیCellular internalization - درونی سازی سلولیStructure-activity relationship - رابطه ساختار-فعالیتStructure activity relationship study - رابطه فعالیت فعالیت ساختاریarea under the curve - منطقه تحت منحنی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The plant-derived decapeptide OSIP108 increases tolerance of yeast and human cells to apoptosis-inducing agents, such as copper and cisplatin. We performed a whole amino acid scan of OSIP108 and conducted structure-activity relationship studies on the induction of cisplatin tolerance (CT) in yeast. The use of cisplatin as apoptosis-inducing trigger in this study should be considered as a tool to better understand the survival-promoting nature of OSIP108 and not for purposes related to anti-cancer treatment. We found that charged residues (Arg, His, Lys, Glu or Asp) or a Pro on positions 4-7 improved OSIP108 activity by 10% or more. The variant OSIP108[G7P] induced the most pronounced tolerance to toxic concentrations of copper and cisplatin in yeast and/or HepG2 cells. Both OSIP108 and OSIP108[G7P] were shown to internalize equally into HeLa cells, but at a higher rate than the inactive OSIP108[E10A], suggesting that the peptides can internalize into cells and that OSIP108 activity is dependent on subsequent intracellular interactions. In conclusion, our studies demonstrated that tolerance/survival-promoting properties of OSIP108 can be significantly improved by single amino acid substitutions, and that these properties are dependent on (an) intracellular target(s), yet to be determined.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Ageing and Development - Volume 161, Part B, January 2017, Pages 247-254
Journal: Mechanisms of Ageing and Development - Volume 161, Part B, January 2017, Pages 247-254
نویسندگان
Sara Verbandt, Sónia Troeira Henriques, Pieter Spincemaille, Peta J. Harvey, Gursimran Chandhok, Vanessa Sauer, Barbara De Coninck, David Cassiman, David J. Craik, Bruno P.A. Cammue, Kaat De Cremer, Karin Thevissen,