کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5504125 | 1400215 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-)
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کلمات کلیدی
PCNALHRObRFSHRob/ob - ob / ob میProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیBody mass - جرم بدنMolecular biology - زیستشناسی مولکولیStar - ستارهLeydig cells - سلولهای LeydigSertoli cells - سلولهای SertoliInfertility - ناباروریwild-type - نوع وحشیnicotinamide adenine dinucleotide phosphate - نیکوتین آمید adenine dinucleotide phosphateSteroidogenic acute regulatory protein - پروتئین حاکم استروئیدوژنیک حادAndrogen Receptor - گیرنده آندروژنیEstrogen receptor - گیرنده استروژنLeptin receptor - گیرنده لپتینfollicle-stimulating hormone receptor - گیرنده هورمون تحریک کننده فولیکولLuteinizing hormone receptor - گیرنده هورمون لوتئینیزاسیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The obesity and its comorbidities, including resistance to leptin, impacts the reproductive function. Testes express leptin receptors in the germ cells and Leydig cells. Then, leptin-deficient animals are obese and infertile. We aimed to evaluate the structure and steroidogenic pathway of the testis of deficient leptin mice. Three months old male C57BL/6 mice (wild-type, WT) and deficient leptin (ob/ob) mice had their testes dissected and prepared for analyses. Compared to the WT group, the ob/ob group showed a greater body mass with smaller testes, and alterations in the germinative epithelium: fewer spermatogonia, spermatocytes, and spermatids. The Sertoli cells and the germ cells showed condensed nuclei and nuclear fragmentation indicating cell death, in agreement with a low expression of the proliferating cell nuclear antigen and a high expression of Caspase3. In the ob/ob group, the sperm was absent in the seminiferous tubules, and the steroidogenic pathway was compromised (low 3Beta hydroxysteroid dehydrogenase and steroidogenic acute regulatory protein). Further, all hormone receptors involved in the testicular function were down expressed (androgen, estrogen, follicle-stimulating, luteinizing, aromatase, and nicotinamide adenine dinucleotide phosphate). In conclusion, the findings indicate significant morphological, hormonal and enzymatic changes in the testis of the ob/ob mice. The shifts in the enzymatic steroidogenic pathway and the enzymes related to spermatic activity support the insights about the failures in the fertility of these animals. The study provides new evidence and contributes to the understanding of how the lack of leptin and obesity might negatively modulate the testicular function leading to infertility.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 119, Issue 5, June 2017, Pages 508-515
Journal: Acta Histochemica - Volume 119, Issue 5, June 2017, Pages 508-515
نویسندگان
Fabiane Ferreira Martins, Marcia Barbosa Aguila, Carlos Alberto Mandarim-de-Lacerda,