کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505207 | 1400263 | 2017 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis and assembly of Hepatitis B virus envelope protein-derived particles in Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Synthesis and assembly of Hepatitis B virus envelope protein-derived particles in Escherichia coli Synthesis and assembly of Hepatitis B virus envelope protein-derived particles in Escherichia coli](/preview/png/5505207.png)
چکیده انگلیسی
Hepatitis B virus (HBV) envelope particles have been synthesized in eukaryotic cells (e.g., mammalian cells, insect cells, and yeast cells) as an HB vaccine immunogen and drug delivery system (DDS) nanocarrier. Many researchers had made attempts to synthesize the particles in Escherichia coli for minimize the cost and time for producing HBV envelope particles, but the protein was too deleterious to be synthesized in E. coli. In this study, we generated deletion mutants of HBV envelope L protein (389 amino acid residues (aa)) containing three transmembrane domains (TM1, TM2, TM3). The ÎNC mutant spanning from TM2 to N-terminal half of TM3 (from 237 aa to 335 aa) was found as a shortest form showing spontaneous particle formation. After the N-terminal end of ÎNC mutant was optimized by the N-end rule for E. coli expression, the modified ÎNC mutant (mÎNC) was efficiently expressed as particles in E. coli. The molecular mass of mÎNC particle was approx. 670 kDa, and the diameter was 28.5 ± 6.2 nm (mean ± SD, N = 61). The particle could react with anti-HBV envelope S protein antibody, indicating the particles exhibited S antigenic domain outside as well as HBV envelope particles. Taken together, the E. coli-derived mÎNC particles could be used as a substitute of eukaryotic cell-derived HBV envelope particles for versatile applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 490, Issue 2, 19 August 2017, Pages 155-160
Journal: Biochemical and Biophysical Research Communications - Volume 490, Issue 2, 19 August 2017, Pages 155-160
نویسندگان
Hao Li, Keisuke Onbe, Qiushi Liu, Masumi Iijima, Kenji Tatematsu, Masaharu Seno, Hiroko Tada, Shun' ichi Kuroda,