کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5505327 1400265 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chaperone-like activity of synthetic polyanions can be higher than the activity of natural chaperones at elevated temperature
ترجمه فارسی عنوان
فعالیت شپرون مانند پلینیون های مصنوعی می تواند بالاتر از فعالیت شاپرن های طبیعی در دمای بالا باشد
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- Chaperone-like activity of synthetic polyanion and natural chaperones was compared.
- The polyanion was more efficient than GroEL-like chaperonin at high temperature.
- Poly(styrene sulfonate) was more efficient than sHSP in protection of GAPDH.
- Protective efficiency of the polyanion is specific for different client protein.

Polyelectrolytes are a prospective tool for protection of proteins against aggregation. We compared synthetic polyanion, poly(styrene sulfonate), and natural chaperones of different types, namely, GroEL-like chaperonin from Pseudomonas aeruginosa phage EL and human small heat shock protein HspB5 (αB-crystallin), in their ability to prevent aggregation of client proteins. At 45 °C, all three agents efficiently suppressed thermal aggregation of phage endolysin. At higher temperatures, HspB5 and poly(styrene sulfonate) also inhibited endolysin aggregation, though polyanion became less efficient than HspB5 at 55 °C and 60 °C. However, the polyanion completely protected another protein, glyceraldehyde-3-phosphate dehydrogenase, even at 60 °C, in contrast to both natural chaperones whose effect disappeared at 50-55 °C. These results provide a platform for the development of artificial chaperones based on synthetic polyelectrolytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 489, Issue 2, 22 July 2017, Pages 200-205
نویسندگان
, , , , ,