کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5505533 1400272 2017 35 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an aptamer-ampicillin conjugate for treating biofilms
ترجمه فارسی عنوان
توسعه یک اتصال دهنده آپتامر-آمپی سیلین برای درمان بیوفیلم ها
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Biofilm formation involves the development of extracellular matrix and initially depends on adherence and tropism by flagellar movement. With the widespread development of antibiotic resistance and tolerance of biofilms, there is a growing need for novel anti-infective strategies. No currently approved medications specifically target biofilms. Aptamers are single-stranded nucleic acid molecules that may bind to their targets with high affinity and affect the target functions. We developed a bifunctional conjugate by linking an aptamer targeting bacterial flagella with ampicillin. We investigated its influence on biofilm prevention and dissolution by ultraviolet-visible spectrophotometry, inverted microscopy, and atomic force microscopy. This conjugate had distinctive antibacterial activity. Notably, the conjugate was more active than either component, and thus had a synergistic effect against biofilms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 483, Issue 2, 5 February 2017, Pages 847-854
نویسندگان
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