کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505534 | 1400272 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transgenic RNAi of BmREEPa in silkworms can enhance the resistance of silkworm to Bombyxmori Nucleopolyhedrovirus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Our previous study has identified a gene, BmREEPa, which affects BmNPV invasion in silkworm cells. In this study, we interfered with BmREEPa in silkworm larvae through transgenic technology and screened BmREEPa-RNAi silkworm strains (RP). We found the mortality in RP was lower than that in Dazao, when silkworm larvae were infected with BmNPV via oral and injection routes. And the expression level of VP39 was lower in RP than in Dazao in the group infected via injection. In the oral infection group, VP39 expression level showed significant reduction at 48Â h post-infection. These results revealed that the anti-BmNPV activity was enhanced in RP, and this enhancement probably presents itself during secondary infection via BVs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 483, Issue 2, 5 February 2017, Pages 855-859
Journal: Biochemical and Biophysical Research Communications - Volume 483, Issue 2, 5 February 2017, Pages 855-859
نویسندگان
Xiao-long Dong, Tai-hang Liu, Wei Wang, Cai-xia Pan, Guo-yu Du, Yun-fei Wu, Malavika Adur, Min-Juan Zhang, Min-Hui Pan, Cheng Lu,