کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5505873 1400280 2017 31 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Autophagy regulates Endothelial-Mesenchymal transition by decreasing the phosphorylation level of Smad3
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Autophagy regulates Endothelial-Mesenchymal transition by decreasing the phosphorylation level of Smad3
چکیده انگلیسی
Transforming growth factor-beta2 (TGF-β2) induces Endothelial-Mesenchymal transition (EndoMT) and autophagy in a variety of cells. Previous studies have indicated that activation of autophagy might decrease TGF-β2 induced EndoMT. However, the precise role remains unclear. In the present study, we found that TGF-β2 could induce EndoMT and autophagy in human retinal microvascular endothelial cells (hRMECs). Activation of autophagy by Rapamycin or Trehalose could reduce the expression of Snail, demonstrating a role of autophagy in regulating Snail production both by transcriptional and post-transcriptional mechanism. Co-immunoprecipitation (CoIP) demonstrated that LC3 co-immunoprecipitated with Smad3 and western blot showed that autophagy inducers, Rapamycin and Trehalose, could decrease the phosphorylation level of Smad3. Therefore, our results demonstrate that autophagy counteracts the EndoMT process triggered by TGF-β2 by decreasing the phosphorylation level of Smad3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 487, Issue 3, 3 June 2017, Pages 740-747
نویسندگان
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