کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505929 | 1400282 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional characterization of the Arabidopsis universal stress protein AtUSP with an antifungal activity
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Functional characterization of the Arabidopsis universal stress protein AtUSP with an antifungal activity Functional characterization of the Arabidopsis universal stress protein AtUSP with an antifungal activity](/preview/png/5505929.png)
چکیده انگلیسی
An antifungal protein, AtUSP protein (At3g53990), was isolated from Arabidopsis thaliana leaves by ion and size chromatography and sequenced by N-terminal sequencing. The AtUSP gene amplified from an Arabidopsis leaf cDNA library was transformed to Escherichia coli to express the AtUSP protein. The recombinant protein inhibited the cell growth of various pathogenic fungal strains. The levels of the AtUSP transcripts were increased by various stresses, including pathogenic infection and salt stress. These results suggest that Arabidopsis AtUSP plays a critical role in the plant tolerance to diverse pathogenic infections. The potent antifungal action, which is a new function of AtUSP, was attributed to fungal reactive oxygen species (ROS) generation and mitochondrial potential alteration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 486, Issue 4, 13 May 2017, Pages 923-929
Journal: Biochemical and Biophysical Research Communications - Volume 486, Issue 4, 13 May 2017, Pages 923-929
نویسندگان
Seong-Cheol Park, Young Jun Jung, Yongjae Lee, Il Ryong Kim, Min-A Seol, Eun-Ji Kim, Mi-Kyeong Jang, Jung Ro Lee,