کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5506497 | 1400297 | 2016 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Siglec-F is a novel intestinal M cell marker Siglec-F is a novel intestinal M cell marker](/preview/png/5506497.png)
- A new monoclonal antibody recognizing Siglec-F has been established.
- Siglec-F is expressed on mouse Peyer's patch microfold (M) cells.
- Siglec-F antibody binds to PP M cells in vivo.
Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell-specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M cells, demonstrating the potential to target M cells via Siglec-F.
Journal: Biochemical and Biophysical Research Communications - Volume 479, Issue 1, 7 October 2016, Pages 1-4