کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5506531 1400298 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of a new sweat gland progenitor population in mice and the role of their niche in tissue development
ترجمه فارسی عنوان
شناسایی جمعیت جدید پروتز ژن عرق در موش و نقش طاقچه آنها در توسعه بافت
کلمات کلیدی
اپیدرم پیشگامی غدد عرق، موقعیت مناسب، جایگاه، توسعه غده شکمی، التیام زخم،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Sweat gland cells are responsible for the regulation of body temperature and are critical for wound repair. Furthermore, they have the regenerative potential in response to injury, and show a substantial turnover during both wound healing and homeostasis. However, as a usual research model of sweat gland, mice have not too much glandular cells for experiments. In this study, we identify previously unreported sweat gland progenitor population in mice and characterize them. The progenitor characteristics of sweat gland were confirmed using cellular immunofluorescence assay and quantitative real-time PCR assay. K8 and K18 expression was barely detected in the early stage of skin development (Embryo 17.5d) and increased to a high level at P5d (postnatal 5d), then showed reduction at adult stage (P28d). Further investigation of K8 and K18 positive cells using tissue immunofluorescence revealed the presence of sweat gland progenitors in back epidermis of mice at early stage of sweat gland development and continuous reduction during the developmental process. In vivo transplantation assay with animal models elucidated that sweat gland specific niche in paw pads was critical for the development of sweat gland cells. Although the relationship between new sweat gland progenitors and their niche still needs to be further investigated, the presence of these cells implicates that there is more source ascribed to sweat glands in addition to serving as progenitors in mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 479, Issue 4, 28 October 2016, Pages 670-675
نویسندگان
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