کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5506773 | 1400303 | 2016 | 7 صفحه PDF | دانلود رایگان |

- cDNA encoding a MW â 59 KDa luciferase isolated from Arachnocampa richardsae.
- A. richardsae luciferase member of Acyl-CoA ligase family.
- Presence of D-luciferin in A. richarsae elucidated by TLC and MS.
- A. richardsae light organs contain â¼7 pmol of D-luciferin.
- A. richardsae luciferase produces blue light upon addition of D-luciferin.
Bioluminescence is the emission of visible light by living organisms. Here we describe the isolation and characterisation of a cDNA encoding a MW â 59,000 Da luciferase from the Australian glow-worm, Arachnocampa richardsae. The enzyme is a member of the acyl-CoA ligase superfamily and produces blue light on addition of D-luciferin. These results are contrary to earlier reports (Lee, J., Photochem Photobiol 24, 279-285 (1976), Viviani, V. R., Hastings, J. W. & Wilson, T., Photochem Photobiol 75, 22-27 (2002)), which suggested glow-worm luciferase has MW â 36,000 Da and is unreactive with beetle luciferin. There are more than 2000 species of firefly, which all produce emissions from D-luciferin in the green to red regions of the electromagnetic spectrum. Although blue-emitting luciferases are known from marine organisms, they belong to different structural families and use a different substrate. The observation of blue emission from a D-luciferin-using enzyme is therefore unprecedented.
Journal: Biochemical and Biophysical Research Communications - Volume 478, Issue 2, 16 September 2016, Pages 533-539