کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5506886 | 1400305 | 2016 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased a-series gangliosides positively regulate leptin/Ob receptor-mediated signals in hypothalamus of GD3 synthase-deficient mice
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کلمات کلیدی
DMEMGD3 synthaseGlcCerUCP-1ObRGangliosideBATRT-PCRSTAT3FBSDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoWAT, White adipose tissue - بافت چربی سفید، بافت ادیپوز سفیدbrown adipose tissue - بافت چربی قهوه ایfetal bovine serum - سرم جنین گاوArc - قوسLeptin - لپتین knockout - ناکاوتwild type - نوع وحشیarcuate nucleus - هسته قوسHypothalamus - هیپوتالاموسreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسuncoupling protein 1 - پروتئین جدا سازی 1WAT - چیglucosylceramide - گلوکوزیلسرامیدReceptor - گیرنده Leptin receptor - گیرنده لپتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Gangliosides are widely involved in the regulation of cells and organs. However, little is known about their roles in adipose tissues and hypothalamus. In GD3 synthase-knockout (GD3S KO) mice, deletion of b-series gangliosides resulted in the reduction of serum leptin due to disturbed secretion from adipocytes. To examine whether leptin signals altered, leptin/leptin receptor (ObR)-mediated signaling in hypothalamus was analyzed. Hypothalamus of GD3S KO mouse showed increased expression of GM1 and GD1a, and increased activation of ObR-mediated signals such as pSTAT3 and c-Fos. Leptin stimulation of hypothalamus-derived N-41 cells and their transfectants with GD3S cDNA showed that a-series gangliosides positively regulate leptin/ObR-mediated signals. Co-precipitation analysis revealed that ObR interacts with a-series gangliosides with increased association by leptin stimulation. In brown adipose tissues (BAT) of GD3S KO mice, their weights and adipocyte numbers were increased, and BAT markers such as PGC1α and UCP-1 were also up-regulated. These results suggested that leptin/ObRb-mediated signals were enhanced in hypothalamus of GD3S KO mice due to increased a-series gangliosides, leading to the apparently similar features of energy expenditure between the KO and wild type mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 479, Issue 3, 21 October 2016, Pages 453-460
Journal: Biochemical and Biophysical Research Communications - Volume 479, Issue 3, 21 October 2016, Pages 453-460
نویسندگان
Shuting Ji, Kyohei Tokizane, Yuki Ohkawa, Yuhsuke Ohmi, Ryoichi Banno, Tetsuya Okajima, Hiroshi Kiyama, Koichi Furukawa, Keiko Furukawa,