کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5509428 | 1538514 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
FOS-1 functions as a transcriptional activator downstream of the C. elegans JNK homolog KGB-1
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کلمات کلیدی
JnkAP-1DAF-16SAPKc-Jun N-terminal kinases - C-Jun N-terminal kinasesMAPK - MAPKanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceactivator protein-1 - پروتئین فعال کننده-1mitogen-activated protein kinase - پروتئین کیناز فعال با mitogenStress-activated protein kinase - پروتئین کیناز فعال شده با استرسAntagonistic pleiotropy - پلینیوتراپی آنتاگونیستCadmium - کادمیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
JNK proteins are conserved stress-activated MAP kinases. In C. elegans, the JNK-homolog KGB-1 plays essential roles in protection from heavy metals and protein folding stress. However, the contributions of KGB-1 are age-dependent, providing protection in larvae, but reducing stress resistance and shortening lifespan in adults. Attenuation of DAF-16 was linked to the detrimental contributions of KGB-1 in adults, but its involvement in KGB-1-dependent protection in larvae remains unclear. To characterize age-dependent contributions of KGB-1, we used microarray analysis to measure gene expression following KGB-1 activation either in developing larvae or in adults, achieved by knocking down its negative phosphatase regulator vhp-1. This revealed a robust KGB-1 regulon, most of which consisting of genes induced following KGB-1 activation regardless of age; a smaller number of genes was regulated in an age-dependent manner. We found that the bZIP transcription factor FOS-1 was essential for age-invariant KGB-1-dependent gene induction, but not for age-dependent expression. The latter was more affected by DAF-16, which was further found to be required for KGB-1-dependent cadmium resistance in larvae. Our results identify FOS-1 as a transcriptional activator mediating age-invariant contributions of KGB-1, including a regulatory loop of KGB-1 signaling, but also stress the importance of DAF-16 as a mediator of age-dependent contributions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 30, January 2017, Pages 1-8
Journal: Cellular Signalling - Volume 30, January 2017, Pages 1-8
نویسندگان
Zhe Zhang, Limeng Liu, Kwame Twumasi-Boateng, Dena H.S. Block, Michael Shapira,