کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510564 | 1539262 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional characterization of BdB1, a well-conserved carboxylesterase among tephritid fruit flies associated with malathion resistance in Bactrocera dorsalis (Hendel)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
There are many evidences that insect carboxylesterase possess important physiological roles in xenobiotic metabolism and are implicated in the detoxification of organophosphate (OP) insecticides. Despite the ongoing resistance development in the oriental fruit fly, Bactrocera dorsalis (Hendel), the molecular basis of carboxylesterase and its ability to confer OP resistance remain largely obscure. This study was initiated to provide a better understanding of carboxylesterase-mediated resistance mechanism in a tephritid pest fly. Here, we narrow this research gap by demonstrating a well-conserved esterase B1 gene, BdB1, mediates malathion resistance development via gene upregulation with the use of a laboratory selected malathion-resistant strain (MR) of B. dorsalis. No sequence mutation of BdB1 was detected between MR and the susceptible strain (MS) of B. dorsalis. BdB1 is predominantly expressed in the midgut, a key insect tissue for detoxification. As compared with transcripts in MS, BdB1 was significantly more abundant in multiple tissues in the MR. RNA interference (RNAi)-mediated knockdown of BdB1 significantly increased malathion susceptibility. Furthermore, heterologous expression along with cytotoxicity assay revealed BdB1 could probably have the function of malathion detoxification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 200, October 2017, Pages 1-8
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 200, October 2017, Pages 1-8
نویسندگان
Luo-Luo Wang, Xue-Ping Lu, Guy Smagghe, Li-Wei Meng, Jin-Jun Wang,