کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510617 | 1539266 | 2017 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of methimazole on Drosophila glucolipid metabolism in vitro and in vivo
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کلمات کلیدی
MMICPTACCFASRT-qPCRP450sS6KFMOsSREBPcytochrome P450 enzymes - آنزیم های سیتوکروم P450AKH - آهacetyl-CoA carboxylase - استیل کروکسی سیلازfatty acid synthase - اسید چرب سنتازtriglyceride - تریگلیسریدMethimazole - متیمزولFlavin-containing monooxygenases - مونواکسیژنازهای حاوی فلاوینDrosophila melanogaster - مگس سرکه، مگس میوهAdipokinetic hormone - هورمون آدپیوکینتیکreal-time quantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استsterol regulatory element binding protein - پروتئین اتصال دهنده عصاره استرولribosomal protein S6 kinase - پروتئین ریبوزومی S6 کینازCarnitine palmitoyltransferase - کارنتین پالمیتیل ترانسفرازGlycogen - گلیکوژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Methimazole (MMI) is an antithyroid agent widely used in the treatment of hyperthyroidism, and metabolized by cytochrome P450 enzymes and flavin-containing monooxygenases in mammals. However, drug overdose and the inadequate detoxification of the metabolite(s) are responsible for hepatocellular damage and organ dysfunction. Depending on the desired properties, Drosophila melanogaster has recently emerged as an ideal model organism for the study of human diseases. Here we investigated the changes in metabolic profiles and mRNA expressions related to glucolipid metabolism in response to treatment with MMI in Drosophila. Remarkable loss of lifespan occurred in fruit flies fed on the diets containing 10 or 30 mM MMI compared to unsupplemented controls. To examine whether MMI affects glucolipid metabolism in vitro and in vivo, fruit flies were fed diets containing 30 mM MMI for two weeks and Drosophila S2 cells were incubated with 300 μM MMI for 48 h. Measurements of metabolites showed that triglyceride content dramatically decreased (30.56% in vivo and 18.13% in vitro), and glycogen content significantly increased (10.7% in vivo and 126.8% in vitro). Quantitative analyses indicated that mRNA expression levels of Dmfmo1, s6k, dilp2, acc and dilp5 genes involved in metabolic homeostasis were remarkably down-regulated in vivo and in vitro. Meanwhile, the addition of MMI could significantly reduce the lipid droplet content in S2 cells by approximately 25% compared to control subjects. These data may provide a biological basis for the study of MMI on disease symptoms and complications, and discovery of therapeutic treatments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 196, June 2017, Pages 54-60
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 196, June 2017, Pages 54-60
نویسندگان
Qi (éçª), Bo (è¡æ³¢), Yuan (èå), David Kibe Mburu, Xian (é¶å¨´), Jianya (è建äº),