کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5511253 1539849 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperStructural analysis of Dioclea lasiocarpa lectin: A C6 cells apoptosis-inducing protein
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Research paperStructural analysis of Dioclea lasiocarpa lectin: A C6 cells apoptosis-inducing protein
چکیده انگلیسی


- DLL three-dimensional structure was determined.
- Molecular docking with mono- and oligosaccharides was performed.
- Antiglioma effect of DLL was evaluated.
- DLL reduced cell viability via caspase 3 activation.

Lectins are multidomain proteins that specifically recognize various carbohydrates. The structural characterization of these molecules is crucial in understanding their function and activity in systems and organisms. Most cancer cells exhibit changes in glycosylation patterns, and lectins may be able to recognize these changes. In this work, Dioclea lasiocarpa seed lectin (DLL) was structurally characterized. The lectin presented a high degree of similarity with other lectins isolated from legumes, presenting a jelly roll motif and a metal-binding site stabilizing the carbohydrate-recognition domain. DLL demonstrated differential interactions with carbohydrates, depending on type of glycosidic linkage present in ligands. As observed by the reduction of cell viability in C6 cells, DLL showed strong antiglioma activity by mechanisms involving activation of caspase 3.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 92, November 2017, Pages 79-89
نویسندگان
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