Polysaccharide from Mesona chinensis: Extraction optimization, physicochemical characterizations and antioxidant activities

- Optimal extraction conditions of MCP were obtained by response surface methodology.
- Physicochemical characterizations of MCP were measured by HPGPC, FTIR and HPAEC.
- MCP exhibited antioxidant activities in a concentration-dependent manner.
- MCP might be a good candidate for further investigations of functional foods.

The optimization of extraction conditions, physicochemical characterizations and antioxidant activities of polysaccharide from Mesona chinensis (MCP) were investigated. Optimal extraction conditions of MCP with the highest yield of 7.05 ± 0.12% was obtained by response surface methodology (RSM). The physicochemical characterizations of MCP obtained at the optimal extraction conditions were detected by TU-1900 spectrophotometer, high performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography (HPAEC) and fourier transform infrared spectroscopy (FT-IR) as well, revealing that MCP was a heteropolysaccharide containing uronic acid (29.3 ± 1.3%) and protein (10.4 ± 0.8%), with an average molecular weight (Mw) of 1.45 × 106 Da, which mainly consisted of galactose (Gal) and glucose (Glc) in a molar ratio of 1.00:1.38. Furthermore, MCP exhibited considerable antioxidant potential on scavenging hydroxyl (53.87 ± 0.44%), superoxide anion (58.42 ± 1.17%) and DPPH radicals (55.59 ± 0.69%). Our results indicated that MCP might be a good candidate for further investigations of functional foods.

218The polysaccharide from Mesona chinensis was extracted by response surface methodology and its physicochemical characterizations and antioxidant activities were investigated.