کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5512510 | 1540225 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A combined biochemical and computational studies of the rho-class glutathione s-transferase sll1545 of Synechocystis PCC 6803
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کلمات کلیدی
CuOOHRMSDGSHGSTRMSFPCA - PCAPrincipal component analysis - تحلیل مولفههای اصلی یا PCAessential dynamics - دینامیک ضروریSynechocystis - سینکوسیستیسMolecular dynamic simulation - شبیه سازی پویایی مولکولیModeling - مدلسازیroot mean square deviation - میانگین انحراف مربع ریشهroot mean square fluctuation - نوسان میانگین مربع ریشهPeroxide - پراکسیدPeroxidase - پراکسیدازreduced glutathione - کاهش گلوتاتیونGlutathione - گلوتاتیونglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A combined biochemical and computational studies of the rho-class glutathione s-transferase sll1545 of Synechocystis PCC 6803 A combined biochemical and computational studies of the rho-class glutathione s-transferase sll1545 of Synechocystis PCC 6803](/preview/png/5512510.png)
چکیده انگلیسی
Peroxides are one of the most important radicals that cause oxidative stress. Certain Glutathione S-transferases (GSTs) have been reported to show peroxidase activity. We report a novel peroxidase activity of Synechocystis GST- sll1545. The recombinant protein was purified to homogeneity and characterized. Low Km (0.109 μM) and high Vmax (0.663 μmol minâ1) values suggest a high preference of sll1545 for cumenehydroperoxide. Disc inhibition assay confirmed the ability of the enzyme to protect cells against peroxide-induced damage. sll1545 has very low sequence and structural similarity with theta and alpha class GSTs that exhibit glutathione-dependent peroxidase activity. Recent data from our laboratory shows that sll1545 is also strongly active against dichloroacetate (DCA), which is a characteristic of zeta class GST. Interestingly, sll1545 shows less than 20% sequence identity with zeta class GST. Molecular dynamic simulation results show that sll1545 was much more structurally different from alpha/theta classes. Our results suggest that sll1545 shows structural variation from zeta, theta/alpha classes of GSTs but have related enzymatic activity. Phylogenetic analysis reveal that sll1545 is evolutionally very distinct from the known GSTs. Overall, the data suggest that Synechocystis sll1545 does not belong to any known GST class and represent a novel GST class, which we have named rho.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 94, Part A, January 2017, Pages 378-385
Journal: International Journal of Biological Macromolecules - Volume 94, Part A, January 2017, Pages 378-385
نویسندگان
Tripti Pandey, Rohit Shukla, Harish Shukla, Amit Sonkar, Timir Tripathi, Arvind Kumar Singh,