کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5512785 | 1540620 | 2017 | 45 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A-type ECG and EGCG dimers inhibit 3T3-L1 differentiation by binding to cholesterol in lipid rafts
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کلمات کلیدی
SREBP1cCHOLTMA-DPHPPARγA-type EGCG dimerMDIPoPCNBD-PEDPH1-palmitoyl-2-oleoyl phosphatidylcholine - 1-پالمیتویل-2-اولئویل فسفاتیدیل کولین1,6-diphenyl-1,3,5-hexatriene - 1،6-دیفنیل-1،3،5-هگزیدریلC/EBPα - C / EBPαMolecular dynamics - دینامیک ملکولی یا پویایی مولکولیDisruption - قطعPOPE - پاپcholesterol - کلسترولLipid rafts - گستره چربی، کلک چربی یا لیپید رفت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present study aimed to explore the underlying mechanisms of epicatechin-3-gallate-(4βâ8, 2βâOâ7)-epicatechin-3-gallate (A-type ECG dimer) and epigallocatechin-3-gallate-(4βâ8, 2βâOâ7)-epigallocatechin-3-gallate (A-type EGCG dimer) involved in their strong inhibitory effects on 3T3-L1 preadipocytes differentiation. In the synthetic “lipid raft-like” liposome, A-type ECG and EGCG dimers incorporated into the liposome with high affinity and decreased the fluidity of the liposome. In 3T3-L1 preadipocytes, A-type ECG and EGCG dimers possibly bonded to lipid rafts cholesterol and disrupted the integrity of lipid rafts, thus exerting their notable inhibitory effects on 3T3-L1 preadipocytes differentiation by suppressing mitotic clonal expansion process and mRNA levels of PPARγ, C/EBPα and SREBP1C. A highly positive correlation between the cholesterol binding capacity of the two dimers and their inhibitory effect on 3T3-L1 preadipocytes differentiation (R2=0.9328) was observed. Molecular dynamics simulation further verified that A-type ECG and EGCG dimers could bond to cholesterol via hydrogen bonding. The results of this study suggested that the disruption of A-type ECG and EGCG dimers on membrane lipid rafts by targeting cholesterol in the lipid rafts was involved in the underlying mechanisms of their strong inhibitory effects on 3T3-L1 preadipocytes differentiation. This broadens the understanding of the molecular mechanisms of polyphenols on modulating and controlling of metabolic dysregulation, particularly adipocyte differentiation, which is a significant risk factor associated with the development of cardiovascular disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 48, October 2017, Pages 62-73
Journal: The Journal of Nutritional Biochemistry - Volume 48, October 2017, Pages 62-73
نویسندگان
Wei Zhu, Xiangyi Deng, Jinming Peng, Bo Zou, Chunmei Li,