کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5513660 1541210 2017 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The application of microbeads to microfluidic systems for enhanced detection and purification of biomolecules
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The application of microbeads to microfluidic systems for enhanced detection and purification of biomolecules
چکیده انگلیسی


- Bead-based microfluidics provide short transportation length and high surface-to-volume ratio.
- Commercial microbeads offer varied, robust and dense surface functionalization.
- A system was described that combines microbeads in a microfluidic system.
- This platform can be a development in miniaturized biosensing and biotechnology assays.
- Enhanced multiplexing, speed and sensitivity can be achieved.

This paper describes microbead-based microfluidic systems. Several aspects of bead assays in microfluidics make them advantageous for bioassays in simple microchannels, including enhanced surface-to-volume ratio, improved molecular recognition reaction efficiency, and the wide range of surface functionalization available with commercial microbeads. Two-level SU-8 molds are used to fabricate PDMS microchannels that can hydrodynamically trap different types of microbeads, with characteristic dimensions of tens of microns. The use of these microbead-based microfluidic systems in the biosensing of antibodies, toxins and nucleic acids, as well as in antibody purification will be presented and discussed in this paper.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 116, 1 March 2017, Pages 112-124
نویسندگان
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