کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5513660 | 1541210 | 2017 | 13 صفحه PDF | دانلود رایگان |

- Bead-based microfluidics provide short transportation length and high surface-to-volume ratio.
- Commercial microbeads offer varied, robust and dense surface functionalization.
- A system was described that combines microbeads in a microfluidic system.
- This platform can be a development in miniaturized biosensing and biotechnology assays.
- Enhanced multiplexing, speed and sensitivity can be achieved.
This paper describes microbead-based microfluidic systems. Several aspects of bead assays in microfluidics make them advantageous for bioassays in simple microchannels, including enhanced surface-to-volume ratio, improved molecular recognition reaction efficiency, and the wide range of surface functionalization available with commercial microbeads. Two-level SU-8 molds are used to fabricate PDMS microchannels that can hydrodynamically trap different types of microbeads, with characteristic dimensions of tens of microns. The use of these microbead-based microfluidic systems in the biosensing of antibodies, toxins and nucleic acids, as well as in antibody purification will be presented and discussed in this paper.
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Journal: Methods - Volume 116, 1 March 2017, Pages 112-124