کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5513710 1541270 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel modified physiologically relevant model for cardiac angiogenesis
ترجمه فارسی عنوان
یک مدل اصلاح فیزیولوژیکی مربوط به آنژیوژنز قلبی را تغییر داد
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- Morphological observation of three cardiac angiogenesis assays
- Cardiac explants matrigel assay acquires higher vascular sprout length and number.
- Cardiac explants matrigel assay acquires higher branch point number and vascular sprout area.
- Cardiac explants matrigel assay acquires higher tip cell and filopodia number.
- Hypoxia promotes vascular sprout formation in cardiac explants matrigel assay.

Angiogenesis assays are important tools for studying both the mechanisms of cardiac angiogenesis and the potential development of therapeutic strategies to ischemic heart diseases. Currently, various assays have been used to quantitate cardiac tubule formation, yet no consensus has been reached regarding a suitable assay for evaluating the efficacy of angiogenic stimulants or inhibitors. Most in vivo angiogenesis assays are complex and difficult to interpret, whereas traditional in vitro angiogenesis models measure only one aspect of this process. To bridge the gap between in vivo and in vitro angiogenesis assays, here, we have developed a novel modified cardiac explants matrigel assay. We observed the morphology of vascular sprouts formed in three forms of cardiac angiogenesis assays then used quantitative image analyses to further compare the morphological features of vascular sprouts formed in two cardiac explants angiogenesis assays. Vascular sprouts formed in the fibronectin group were less and short, whereas those formed in the matrigel group were significantly longer, consisting of more area and branch points. Moreover, we found the benefits of this matrigel model by observing the ability of cardiac explants to form vascular sprouts under normoxia or hypoxia condition in the presence of angiogenic stimulant and inhibitor, VEGF and PEDF. In summary, the above analyses revealed that the morphology of vascular sprouts formed in this model appears more representative of myocardial capillary formation in vivo, and this accessible, reliable angiogenic assay is a more physiologically relevant assay which allows further assessment of pharmacologic compounds on cardiac angiogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microvascular Research - Volume 114, November 2017, Pages 84-91
نویسندگان
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