کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5513776 | 1541272 | 2017 | 6 صفحه PDF | دانلود رایگان |
- Simvastatin (SIM) was loaded in PLGA NPs, then, conjugated with mZD7349 peptide to direct against VCAM-1.
- SIM treatment showed increased phosphor - eNOS (Ser1177) expression.
- p- eNOS expression in activated HUVECs treated with conjugated NPs was better those treated with unconjugated NPs.
- The results suggest that conjugated NPs may be usable as an appropriate drug carrier for restoring endothelial dysfunction.
Endothelial dysfunction is initial and critical step of atherosclerosis. Impaired bioavailability of endothelial nitric oxide synthase (eNOS) is one of the main reasons of endothelial dysfunction. Improving bioavailability of eNOS by increasing its expression or activity using statins is an effective therapeutic strategy in restoring endothelial dysfunction.In this study, simvastatin (SIM) as a poorly water-soluble drug was loaded in poly (lactic-co-glycolic acid) (PLGA) nanoparticles (SIM-PLGA-NPs). NPs were then conjugated with mZD7349 peptide (mZD7349-SIM-PLGA-NPs) and directed against vascular cell adhesion molecule 1 (VCAM-1). In vitro evaluation of the NPs for targeted delivery of SIM was performed on activated Human Umbilical Cord Vascular Endothelial Cells (HUVECs) by tumor necrosis factor alpha (TNF-α). Effect of mZD7349-SIM-PLGA-NPs and SIM-PLGA-NPs was compared on eNOS phosphorylation (ser-1177).Results of western blot showed SIM post-treatment increased significantly phosphor-eNOS (Ser1177) expression but no total eNOS expression. The study showed that mZD7349-SIM-PLGA-NPs have particle size, zeta potential value, polydispersity index (PDI) and encapsulation efficacy % of 233 ± 18 nm, â 9.6 ± 1.1 mV, 0.59 ± 0.066 and 69 ± 17.3%, respectively. Also phosphor-eNOS (Ser1177) expression in activated HUVECs treated with mZD7349-SIM-PLGA-NPs was significantly (p < 0.05) better than treated cells with SIM-PLGA-NPs.The results suggest that mZD7349-SIM-PLGA-NPs may be usable as an appropriate drug carrier for restoring endothelial dysfunction.
Journal: Microvascular Research - Volume 112, July 2017, Pages 14-19