کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5515397 1541906 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research articleComparative metabolome analysis of wheat embryo and endosperm reveals the dynamic changes of metabolites during seed germination
ترجمه فارسی عنوان
تحقیقاتی تحلیل متابولومی نسبی جنین و اندوسپرم گندم نشانگر تغییرات دینامیکی متابولیت ها در طول جوانه زنی بذر
کلمات کلیدی
گندم، جوانه زنی دانه، متابولیت ها، جنین، آندوسپرم
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
چکیده انگلیسی


- The embryo mobilises protein and lipid while the endosperm mobilises storage starch and minor protein.
- The primary energy was generated mainly in the embryo by glycolysis during seed imbibition.
- The embryo increased nucleotides, indicating more active transcription and translation metabolisms.

In this study, we performed the first comparative metabolomic analysis of the wheat embryo and endosperm during seed germination using GC-MS/MS. In total, 82 metabolites were identified in the embryo and endosperm. Principal component analysis (PCA), metabolite-metabolite correlation and hierarchical cluster analysis (HCA) revealed distinct dynamic changes in metabolites between the embryo and endosperm during seed germination. Generally, the metabolite changes in the embryo were much greater than those in the endosperm, suggesting that the embryo is more active than the endosperm during seed germination. Most amino acids were upregulated in both embryo and endosperm, while polysaccharides and organic acids associated with sugars were mainly downregulated in the embryo. Most of the sugars showed an upregulated trend in the endosperm, but significant changes in lipids occurred only in the embryo. Our results suggest that the embryo mobilises mainly protein and lipid metabolism, while the endosperm mobilises storage starch and minor protein metabolism during seed germination. The primary energy was generated mainly in the embryo by glycolysis during seed imbibition. The embryo containing most of the genetic information showed increased nucleotides during seed germination process, indicating more active transcription and translation metabolisms.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 115, June 2017, Pages 320-327
نویسندگان
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