Research articleThe wheat mutant DELLA-encoding gene (Rht-B1c) affects plant photosynthetic responses to cadmium stress

- Cd toxicity is alleviated in the Rht-B1c mutant compared to Rht-B1a wild type wheat.
- The Rht-B1c mutation leads to less reduction of pigment content under Cd stress.
- PSI and PSII photosynthetic activities are more Cd-resistant in the Rht-B1c mutant.
- Causes of the resilience of the photosynthetic apparatus in the mutant are discussed.

Тhe sensitivity to cadmium (Cd) stress of two near-isogenic wheat lines with differences at the Rht-B1 locus, Rht-B1a (tall wild type, encoding DELLA proteins) and Rht-B1c (dwarf mutant, encoding modified DELLA proteins), was investigated. The effects of 100 μM CdCl2 on plant growth, pigment content and functional activity of the photosynthetic apparatus of wheat seedlings grown on a nutrient solution were evaluated through a combination of PAM chlorophyll fluorescence, oxygen evolution, oxidation-reduction kinetics of P700 and 77 K fluorescence. The results showed that the wheat mutant (Rht-B1c) was more tolerant to Cd stress compared to the wild type (Rht-B1a), as evidenced by the lower reductions in plant growth and pigment content, lower inhibition of photosystem I (PSI) and photosystem II (PSII) photochemistry and of the oxygen evolution measured with Clark-type and Joliot-type electrodes. Furthermore, the enhanced Cd tolerance was accompanied by increased Cd accumulation within mutant plant tissues.The molecular mechanisms through which the Rht-B1c mutation improves plant tolerance to Cd stress involve structural alterations in the mutant photosynthetic membranes leading to better protection of the Mn cluster of oxygen-evolving complex and increased capacity for PSI cyclic electron transport, protecting photochemical activity of the photosynthetic apparatus under stress. This study suggests a role for the Rht-B1c-encoded DELLA proteins in protective mechanisms and tolerance of the photosynthetic apparatus in wheat plants exposed to heavy metals stress.

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