کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5515758 1542026 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cell-specific expression of plant nutrient transporter genes in orchid mycorrhizae
ترجمه فارسی عنوان
بیان خاصی از سلول ژن های انتقال دهنده گیاهی گیاه در میکوریزا ارکیده
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
چکیده انگلیسی


- An LMD approach was used to study cell-specific expression in orchid mycorrhiza.
- Orchid N transporters were differentially expressed in microdissected cells.
- Transcript levels changed in cells with coils at different developmental stages.
- New functional markers for the orchid cells containing the fungus were identified.

Orchid mycorrhizal protocorms and roots are heterogeneous structures composed of different plant cell-types, where cells colonized by intracellular fungal coils (the pelotons) are close to non-colonized plant cells. Moreover, the fungal coils undergo rapid turnover inside the colonized cells, so that plant cells containing coils at different developmental stages can be observed in the same tissue section. Here, we have investigated by laser microdissection (LMD) the localization of specific plant gene transcripts in different cell-type populations collected from mycorrhizal protocorms and roots of the Mediterranean orchid Serapias vomeracea colonized by Tulasnella calospora. RNAs extracted from the different cell-type populations have been used to study plant gene expression, focusing on genes potentially involved in N uptake and transport and previously identified as up-regulated in symbiotic protocorms. Results clearly showed that some plant N transporters are differentially expressed in cells containing fungal coils at different developmental stages, as well as in non-colonized cells, and allowed the identification of new functional markers associated to coil-containing cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 263, October 2017, Pages 39-45
نویسندگان
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