کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5516109 | 1542310 | 2017 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Purified Stx and λ phage initiator O proteins bind specifically to two different origins of replication in vitro Purified Stx and λ phage initiator O proteins bind specifically to two different origins of replication in vitro](/preview/png/5516109.png)
- Improved method for overexpression of λ o gene and purification of its protein has been developed.
- O proteins of Shiga toxin-converting phage P27 and 933W were purified for the first time.
- All three proteins have been demonstrated to be active in binding to origin regions of bacteriophage λ and P27 genomes.
The O protein is a crucial factor initiating the DNA replication of lambdoid bacteriophage. Efficient DNA replication of Shiga toxin-converting phage is necessary for effective production of Shiga toxin - main virulence factor of STEC strains. We developed an improved protocol for overproduction, bacterial cell lysis and purification of λO protein. With use of this method we have also isolated O proteins of Stx-phage P27 and 933W that were never purified before. Purified proteins were tested for their DNA binding activity and revealed a sequence specific interactions.
Journal: Protein Expression and Purification - Volume 131, March 2017, Pages 16-26