کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5521478 1545313 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperDetermination of nanostructures and drug distribution in lipid nanoparticles by single molecule microscopy
ترجمه فارسی عنوان
تعیین نانوساختارها و توزیع دارو در نانوذرات لیپید با استفاده از میکروسکوپ تک مولکولی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی

Drug loading capacity in nanostructured lipid carriers (NLC) depends on the formation of nanostructures within the lipid matrix. However, investigation of these nanostructures with sizes below the diffraction limit of visible light is quite challenging. Thus, until now the determination of structures and drug distribution within NLCs was not possible. Therefore, we aimed at developing a method to visualize the nanostructures within the lipid carriers. Model NLCs loaded with a lipophilic fluorescent drug mimetic, ATTO-Oxa12, were produced and investigated by single-molecule tracking and localization-based superresolution microscopy. Results revealed spherical ATTO-Oxa12-filled nanostructures with diameters of ∼70 nm and 120-130 nm, both smaller than the NLC size (∼160 nm). The ATTO-Oxa12 diffusion constant was calculated from the single-molecule traces (D ⩾ 1 μm2/s) and indicated the distribution of the model drug in the oily component. Together these data suggest the existence of drug-loaded oily nanocompartments, which could fill up to ∼50% of the model NLCs' volume. In conclusion, a novel tool based on single-molecule microscopy is now available that allows for the precise determination of drug distribution and the characterization of lipid nanostructures, information that is paramount for optimizing lipid nanoparticle formulations.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 110, January 2017, Pages 31-38
نویسندگان
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