کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5538971 1552807 2017 18 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm
چکیده انگلیسی
Short-term storage of semen is a useful strategy for preservation of fish spermatozoa in reproductive management, however, no information is available on Patagonian blenny (Eleginops maclovinus) semen. This study was designed to evaluate the effects of in vitro storage on sperm function parameters in Patagonian blenny. Samples of undiluted (C: control) and diluted semen 1:1 v/v (DS1) and 1:3 v/v (DS2) in Storfish® were stored at 4 °C for 7 days. Motility, viability, mitochondrial membrane potential (ΔΨMit), DNA fragmentation and ATP content were assessed at 0, 3 and 7 days. The results demonstrated that sperm function parameters were affected significantly by the time of storage, and that dilution allows better preservation of sperm motility. On the third day of storage, spermatozoa showed a motility > 56% in DS1 and DS2, nevertheless, in C it significantly decreased as well as its ATP content (P < 0.05), whereas viability was maintained > 70% and ΔΨMit > 45%. However, a significant decline in the percentage of sperm function parameters (P < 0.05) was observed at 7 days of storage for all samples of undiluted and diluted semen. DNA fragmentation showed no significant increase in all groups, demonstrating < 5% at day 7. In vitro storage conditions maintain better sperm motility in diluted than undiluted semen, preserving the sperm function in all groups assessed during 3 days. In conclusion, the results provide new data on Patagonian blenny sperm quality with respect to in vitro short-term storage evaluated by flow cytometry, this information must be considered for implementing in aquaculture reproductive strategies for the conservation of this species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 481, 1 December 2017, Pages 58-63
نویسندگان
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