کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5539110 | 1552806 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Novel method for induced propagation of fish: Sperm injection in oviducts and ovary/ovarian lavage with sperm
ترجمه فارسی عنوان
روش جدید برای انتشار القاء شده ماهی: تزریق اسپرم در تخم مرغ و تخمیر تخمک / تخمدان با اسپرم
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کلمات کلیدی
بازتولید مصنوعی، لقاح آزمایشگاهی، تخم ریزی منجر شده، کپور معمولی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
چکیده انگلیسی
Motility of spermatozoa in most freshwater teleost species is suppressed by the osmolality of the seminal plasma in the testes, sperm ducts and initiated by a decrease of osmolality upon spawning in fresh water. We hypothesized that, similarly to internally fertilising fish species, spermatozoa of freshly stripped sperm, when injected directly into the ovary through the oviduct, will remain inactivated and maintain its fertilisation capacity for extended periods. Fertilisation is expected to occur after ovulated oocytes with spermatozoa on their surfaces are released into the water. To test this hypothesis, experiments were carried out on carp (Cyprinus carpio) as a model fish. Stripped and pooled sperm samples were injected by catheter into the ovarian cavity through the oviduct 2Â h and 12Â h before expected spawning. To prevent any loss of gametes the genital area of treated females were stitched by a standard protocol used in large-scale hatchery systems. At ovulation, eggs were stripped and fertilised with salt-carbamide solution. All sperm-injected females produced some fertilised eggs that developed normally while fertilisation rates varied considerably (24.2-81.2%). This proof of principle experiment demonstrates the potential for routine use as alternative to in vitro fertilisation for the propagation of fish species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 482, 1 January 2018, Pages 124-129
Journal: Aquaculture - Volume 482, 1 January 2018, Pages 124-129
نویسندگان
Tamás Müller, László Horváth, Tamás Szabó, István Ittzés, Attila Bognár, Petra Faidt, Áron Ittzés, Béla Urbányi, Balázs Kucska,