کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5539954 | 1553559 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
γδ T cells represent a major spontaneously cytotoxic cell population in the chicken
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: γδ T cells represent a major spontaneously cytotoxic cell population in the chicken γδ T cells represent a major spontaneously cytotoxic cell population in the chicken](/preview/png/5539954.png)
چکیده انگلیسی
Natural killer cells in the chicken are mainly confined to the intestine, while only small frequencies are detectable in spleen, lung and blood. Here, we compared the spontaneous cytotoxicity of lymphocytes isolated from blood, spleen and intestine using a flow cytometric based cytotoxicity assay. There was no spontaneous cytotoxicity detected in chicken blood preparations. In contrast, freshly prepared splenocytes exhibited a spontaneous cytotoxicity of up to 50% and intestinal epithelial lymphocytes of up to 85%. This cytotoxicity was observed against the RP9 but not against the chicken CU24 target cell line. The observed cytotoxicity was MHC unrestricted since B2B2 derived effector cells killed RP9 target cells (B2B15) equally well compared to MHC mismatched 2D8 targets (B19B19). The cytotoxicity of splenocytes was enhanced by preincubation with IL-2 or strongly increased with IL-2 plus IL-12. By cell sorting, we identified the CD8+γδ T cell subset as the major effectors, whereas both CD8âγδ T cells and CD8+αβ T cells had only low cytolytic potential. Within intestinal lymphocyte CD45+cells displayed cytotoxicity as well as sorted γδ T cells and NK cell. In conclusion, the chicken γδ T cells represent a major cytotoxic lymphocyte subset that can lyse target cells in a MHC unrestricted manner.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 73, August 2017, Pages 175-183
Journal: Developmental & Comparative Immunology - Volume 73, August 2017, Pages 175-183
نویسندگان
Lisa Fenzl, Thomas W. Göbel, Marie-Luise Neulen,