کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5544720 | 1554997 | 2017 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of JAK-STAT signaling pathway genes and their microRNAs in the intestinal mucosa of genetically disparate chicken lines induced with necrotic enteritis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
miRNAsOSMLIFERKTyk2PIASCNFp38MAPKJAK-STAT signalingSOCSPRLEPO-RJanus kinase - کیناز جانوس v-akt murine thymoma viral oncogene homolog - v-active ویروس آنفلوانزای ویروسی ویروسی tymomaSTAT - آمارAkt - آکتnecrotic enteritis - انتروت نکروتیکoncostatin M - اوکتواستاتین مinterferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینtyrosine kinase 2 - تیروزین کیناز 2reverse transcription - رونویسی معکوسsuppressor of cytokine signaling - سرکوب کننده سیگنالینگ سیتوکینcolony stimulating factor - عامل تحریک کننده کلنیleukemia inhibitory factor - عامل مهارکننده لوکمیCSF - مایع مغزی نخاعیSignal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیprotein inhibitor of activated Stat - مهارکننده پروتئین فعال شدهChicken - مُرغ خانگیGrowth hormone - هورمون رشدp38 mitogen activated protein kinase - پروتئین کیناز فعال میتوکندر P38Prolactin - پرولاکتین JAK - چگونهextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیErythropoietin Receptor - گیرنده اریتروپویتینthrombopoietin receptor - گیرنده ترومبوپوئیتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The JAK-STAT signaling pathway plays a key role in cytokine and growth factor activation and is involved in several cellular functions and diseases. The main objective of this study was to investigate the expression of candidate JAK-STAT pathway genes and their regulators and interactors in the intestinal mucosal layer of two genetically disparate chicken lines [Marek's disease (MD)-resistant line 6.3 and MD-susceptible line 7.2] induced with necrotic enteritis (NE). Through RNA-sequencing, we investigated 116 JAK-STAT signaling pathway-related genes that were significant and differentially expressed between the intestinal mucosa of the two lines compared with respective uninfected controls. About 15 JAK-STAT pathway genes were further verified by qRT-PCR, and the results were in agreement with our sequencing data. All the identified 116 genes were annotated through Gene Ontology and mapped to the KEGG chicken JAK-STAT signaling pathway. To the best of our knowledge, this is the first study to represent the transcriptional analysis of a large number of candidate genes, regulators, and potential interactors in the JAK-STAT pathway of the two chicken lines induced with NE. Several key genes of the interactome, namely, STAT1/3/4, STAT5B, JAK1-3, TYK2, AKT1/3, SOCS1-5, PIAS1/2/4, PTPN6/11, and PIK3, were determined to be differentially expressed in the two lines. Moreover, we detected 68 known miRNAs variably targeting JAK-STAT pathway genes and differentially expressed in the two lines induced with NE. The RNA-sequencing and bioinformatics analyses in this study provided an abundance of data that will be useful for future studies on JAK-STAT pathways associated with the functions of two genetically disparate chicken lines induced with NE.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 187, May 2017, Pages 1-9
Journal: Veterinary Immunology and Immunopathology - Volume 187, May 2017, Pages 1-9
نویسندگان
Anh Duc Truong, Deivendran Rengaraj, Yeojin Hong, Cong Thanh Hoang, Yeong Ho Hong, Hyun S. Lillehoj,