کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5544768 | 1555002 | 2016 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of an autologous canine cancer vaccine system for resectable malignant tumors in dogs
ترجمه فارسی عنوان
توسعه یک سیستم واکسن سرطان سگ سلولی برای تومورهای بدخیم قابل تنظیم در سگ ها
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کلمات کلیدی
RIPArhGM-CSFLimulus Amebocyte LysateLALSBATSBoligodeoxynucleotideODNAPCPAGEADCCPBSantibody-dependent cellular cytotoxicity - آنتی بادی-وابسته به سمیت سلولی سلولیantigen-presenting cell - آنتیژن ارائه سلولAutologous - اتولوگ polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدCancer - سرطانNSCLC - سرطان ریوی غیر سلول کوچکNon-small cell lung cancer - سرطان غیر سلول کوچک ریهDendritic cells - سلول های دندریتیکCanine - سگtrypticase soy broth - سیب سویاPhosphate buffered saline - فسفات بافر شورVaccine - واکسنAntigen - پادگِن یا آنتیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
While conventional therapies exist for canine cancer, immunotherapies need to be further explored and applied to the canine setting. We have developed an autologous cancer vaccine (K9-ACV), which is available for all dogs with resectable disease. K9-ACV was evaluated for safety and immunogenicity for a variety of cancer types in a cohort of companion dogs under veterinary care. The autologous vaccine was prepared by enzymatic digestion of solid tumor biopsies. The resultant single cell suspensions were then UV-irradiated resulting in immunogenic cell death of the tumor cells. Following sterility and endotoxin testing, the tumor cells were admixed with CpG ODN adjuvant and shipped to the participating veterinary clinics. The treating veterinarians then vaccinated each patient with three intradermal injections (10 million cells per dose) at 30-day intervals (one prime and two boost injections). In a cohort of 20 dogs completing the study, 17 dogs (85%) developed an augmented IgG response to autologous tumor antigens as demonstrated using western blot analysis of pre- and post-peripheral blood samples. We also report several dogs have lived beyond expected survival time based on previously published data. In summary, K9-ACV is an additional option to be considered for the treatment of dogs with resectable cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 182, December 2016, Pages 95-100
Journal: Veterinary Immunology and Immunopathology - Volume 182, December 2016, Pages 95-100
نویسندگان
J.R. PhD, R. BVSc, T.J. BA, M.G. MS, D.A. PhD,